THE 2-FDCK HCL DIARIES

The lab claims fixation will induce the dyes to leak within the cells. I believe the assumption is usually that fixation may well hurt cell membranes. I did discover a paper which describes fixation employing methanol or acetic acid, after staining with a DCFDA derivative. DCFDA would be the reagent that stains ROS in the kit ab113851, and I think